Bioassays

enzymes

G-Biosciences offers a range of enzymes.

LongLife™ Enzyme Preparations
Enzymes regularly used in laboratory applications often require preparation of fresh solution before each use. Making fresh enzyme solution for each application is time consuming and wasteful. A wide variety of enzyme preparations in a ready-to-use format are offered, including:

LongLife™ Zymolyase® for the digestion of yeast and fungal cell walls
LongLife™ Lysozyme for the digestion of bacterial cell walls
LongLife™ PE LB Lysozyme for the digestion of bacterial cell walls and fully compatible with the PE LB buffer system. Reduces viscosity build-up due to presence of nucleases.
LongLife™ Proteinase K for the digestion of proteins in nucleic acid preparations.
LongLife™ Nuclease for the removal of nucleic acids. LongLife™ RNase for the digestion of RNA.
LongLife™ DNase for the digestion of DNA.

LongLife™ enzyme preparations have a long shelf life and no weighing or buffer preparation is needed; simply take an aliquot and add in your sample. LongLife™ enzyme preparations contain cofactors necessary for optimal enzymatic activity. Supplied in suspension form and when stored properly have a one year shelf life.
LongLife™ Enzyme Preparations Product Details | Order Online

Recombinant Human Caspases
Recombinant human caspases 1-3 and 6-10 are supplied lyophilized in quantities of 25 units.

One unit of the specific recombinant caspase is the enzyme activity that cleaves 1nmol of a specific caspase substrate per hour at 37ºC in a reaction solution containing 50mM HEPES (pH7.2), 50mM NaCl, 0.1% CHAPS, 10mM EDTA, 5% Glycerol and 10mM DTT.
Recombinant Human Caspases Product Details | Order Online

Thiopurine S-methyltransferase (EC 2.1.1.67)
Methylation of key biological molecules and proteins plays important roles in numerous biological systems, including signal transduction, biosynthesis, protein repair, gene silencing and chromatin regulation.

The S-adenosylmethionine (SAM) dependent methyltransferases use SAM, the second most commonly used enzymatic cofactor after ATP. SAM, also known as AdoMet, acts as a donor of a methyl group that is required for the modification of proteins and DNA. Aberrant levels of SAM have been linked to many abnormalities, including Alzheimer’s, depression, Parkinson’s, multiple sclerosis, liver failure and cancer.

Thiopurine S-methyltransferase transfer methyl groups for S-adenosylmethionine (SAM) to thiopurines.

This enzymes is a highly purified recombinant human Thiopurine S-methyltransferase, EC 2.1.1.67.
Thiopurine S-methyltransferase (EC 2.1.1.67) Product Details | Order Online

Adenine Deaminase (EC 3.5.4.2)
Adenine Deaminase (EC 3.5.4.2) converts Adenine to Hypoxanthine. A highly purified recombinant enzyme protein supplied at a 1µM concentration.
Adenine Deaminase (EC 3.5.4.2) Product Details | Order Online

Adenosylhomocysteine nucleosidase (EC 3.2.2.9)
Adenosylhomocysteine nucleosidase (EC 3.2.2.9) converts S-adenosylhomocysteine (AdoHcy) to S-(deoxy-D-ribos-5-yl)-L-homocysteine + adenine. A highly purified recombinant enzyme protein supplied at a 10µM concentration.
Adenosylhomocysteine nucleosidase (EC 3.2.2.9)Product Details | Order Online