Protein Quantification Assays
G-Biosciences offers a range of assays for the quantification of proteins.
NI™ (Non-Interfering™) Protein Assay
A highly sensitive, colorimetric protein assay that overcomes interference of common laboratory agents present in protein solutions and shows minimal protein-to-protein variation.
The assay is unaffected by the presence of common laboratory agents, such as reducing agents, chelating agents, detergents, amines, sugars, chaotropes, salts, drugs, antibiotics, cobalt and other common laboratory agents. The NI™ Protein Assay is composed of two simple steps: Universal Protein Precipitating Agent (UPPA™) is added to the protein solutions to rapidly precipitate total protein. Protein is immobilized by centrifugation and interfering agents in the supernatant are discarded.
Protein concentration is assayed by mixing with an alkaline solution containing a known concentration of copper salt; the copper ions bind to the peptide backbone and the assay measures the unbound copper ions. The assay is independent of protein side chains minimizing protein-to-protein variation. The color density is inversely proportional to the amount of protein.
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CB X™ Protein Assay
Protein assays are routinely used in many research fields to estimate proteins in a vast array of buffers and conditions. A major problem for researchers is to select a protein assay from the vast selection on the market that is compatible with their protein sample. CB-X™ Protein Assay eliminates this problem as it is designed to be compatible with all commonly used buffers and conditions in protein isolation, storage and assays.
For protein samples in simple, uncomplicated aqueous buffers CB-X™ is a highly sensitive, single reagent assay that can be performed in 5 minutes. CB-X™ Protein Assay uses a protein dye that is an improvement on the Bradford Coomassie dye reagent.
For complicated protein samples CB-X™ Protein Assay is supplied with reagents to clean up the samples and remove all reagents and chemicals that interfere with accurate protein estimation. These reagents include detergents, chaotropes, reducing agents, alkylating agents, sugars, high salt concentrations, stage and subsequent protein assay is performed in a single tube to ensure no protein loss and to maintain the accuracy of the assay.
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CB™ Protein Assay
An improved Coomassie Dye based protein assay based on the Bradford Protein Assay. This assay is suitable for the simple and rapid estimation of protein concentration. This assay is based on a single Coomassie dye based reagent.
The binding of protein to the dye results in a change of color from brown to blue. The change in color density is proportional to protein concentration. Protein estimation can be performed using as little as 0.5µg protein CB™ Protein Assay is supplied with a simple to follow protocol. Ready to use reagent that does not require prefiltering or dilution. Simply mix the protein solution with CB™ Protein Dye and read optical density. The protein-dye complexes reach a stable end point in 5 minutes.
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SPN™ Protein Assays
A fast and efficient spin column assay. Add the protein sample to the SPN™ spin columns and wash to remove non-protein agents, including detergents and chaotropes. Next, add the protein dye and spin to remove free dye. After a second brief wash, elute the protein bound dye with the supplied elution buffer and measure the optical density of the dye. The concentration of protein is determined by comparing the optical density data to the supplied reference data. No protein standards are required. Protein concentration can be determined in less than 10 minutes.
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Protein dotMETRIC™ Assay
Rapidly, mix 1µl protein sample with the supplied Dilution Buffer and apply 1µl of the solution to the test strip by point-of-contact capillary action. Under the assay's specific buffer conditions the protein enters into the matrix of the test strip, binds and saturates as protein diffuses in a circular manner. Develop the test strip in approximately 5 minutes.
A circular protein spot is produced. The diameter of the spot is proportional to the concentration. By measuring the diameter of the spots with the Protein dotMETRIC™ scale you can easily determine concentration of protein. No expensive spectrophotometers or cuvettes required.
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Protn-Latex™
Protn-Latex™ is a simple and reliable method for estimation of protein contamination in latex. Protein estimation can be performed either on the production site or in any laboratory with a minimum of skill or instrumentation. The test detects as low as 25-40µg protein /gm latex (or 25-50 parts per million).
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