Bioassays
protease assays & screening systems
G-Biosciences offers a selection of assays to detect and screen for proteases in biological solutions.
ProteSEEKER™
ProteSEEKER™ identifies specific types of proteases with a panel of twelve protease inhibitors and a sensitive colorimetric protease screening assay.
ProteSEEKER™ allows researchers to screen their protein samples and establish 
which specific class of proteases are present and therefore design a highly specific protease inhibitor cocktail using the minimal number of protease inhibitors. Alternatively, ProteSEEKER™ can be used to test existing protease inhibitor cocktails and identify their inadequacies and therefore supplement in additional protease inhibitors.
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Fluoro™ Protease Assay
The Fluoro™ Protease Assay Kit is designed for the quantitative determination of proteases present in a protein sample. The assay uses fluorescein isothiocyanate (FITC)-labeled casein as a general protease substrate. The fluorescein label on the FITC-casein is highly quenched. When the proteases present in the sample of interest digest the FITC-casein substrate into smaller peptides, the quenching of the fluorescence label is relieved and the fluorescence of the substrate is increased.
The fluorescence of the FITC-labeled peptide is measured with excitation at 485nm and emission at 535nm to determine protease activity. The kit detects picogram level of proteases present in the sample. The kit is supplied with our chemically stabilized MSG-Trypsin™ for use as a general protease control; however, other specific protease standard controls can be used. MSG-Trypsin™ is an ultra-pure trypsin from porcine pancreas, modified by methylation followed by TPCK treatment and is extremely resistant to autolysis. The kit components are sufficient for 1,000 assays in a microtiter plate format.
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Protease Assay Kit
The Protease Assay Kit is designed for the quantitative determination of proteases present in a protein sample, using a dye-labeled protein substrate.
The proteases present in the sample of interest will digest the protein substrate and release dye labeled peptides. The absorbance of the dye-labeled peptide is measured at 570nm for determination of protease activity.
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Protease Screening Kit
The Protease Screening Kit provides you with a simple and quick method for testing your samples for proteolysis. Simply incubate your sample in the reagent provided and obtain results. The kit uses dye-labeled protein conjugate as protease substrate, which allows nanogram level detection. The absorbance of dye-labeled peptide is measured at 574nm for determination of protease activity. The kit is sufficient for 50 assays in a micro well format.
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Protease Assay Substrates
RESORUFIN-CASEIN PROTEASE SUBSTRATE
A colorimetric substrate that when treated with proteases releases resorufin that has an absorbance of 570nm. Supplied lyophilized.
FITC-CASEIN PROTEASE SUBSTRATE
A fluorescent (fluorescein isothiocyanate (FITC)) substrate that when treated with proteases releases FITC that has an excitation at 485nm and emission at 535nm. Supplied lyophilized.
Protease Assay Substrates Product Details | Order Online
Protease Inhibitor Set
Contains 12 ready-to-use individual protease inhibitors for characterization of protease activity.
