Molecular Biology Products | Plasmid DNA Tools
A selection of products for the isolation, transformation and high throughput screening of plasmids.
plasmid dna tools
Z-Competent™ E.coli Transformation
The Z-Competent™ E. coli Transformation kit is designed to generate competent E. coli cells for simple and highly efficient E. coli transformation. E. coli are grown in SOB medium (supplied with Cat. # GZ-5), washed and suspended in our supplied Competent Buffer. The bacterial cells are now ready for transformations. The transformation efficiency is 2x108-1x109 transformants per μg of pUC19 plasmid. The efficiency of transformation varies depending on E. coli strain and plasmid size.
The competent cells can be used immediately or stored for later use. The kit is supplied with reagents sufficient for processing up to 300 x 100μl aliquots of competent cells for transformations (Cat. # GZ-4) or supplied with reagents and SOB culture media sufficient for processing up to 100 x 100μl aliquots of competent cells (Cat. # GZ-5).
Z-Competent™ E.coli Transformation Product Details | Order Online
plasmid isolation
Plasmid Isolation
Spin column format plasmid isolation kits available in three sizes:
- GET™ Plasmid DNA Miniprep for up to 20µg DNA from 1-5ml culture
- GET™ Plasmid DNA Mediprep for 25-100µg DNA from 25-200ml culture
- GET™ Plasmid DNA Maxiprep for up to 500µg DNA from 100-500ml culture.
Plasmid Isolation Product Details | Order Online
GET™ Plasmid DNA Miniprep
Isolates high quality plasmid DNA from 1-5ml E. coli cultures. The kit utilizes an enhanced DNA binding column to produce high yields of plasmid. This quick and easyprotocol eliminates toxic phenol/ chloroform extractions or lengthy ethanol precipitations. On completion of the protocol, the plasmid DNA is ready for restriction enzyme digestion, sequencing, subcloning and in vitro transcription. Plasmid yields are typically up to 20µg/prep.
GET™ Plasmid DNA Miniprep Product Details | Order Online
GET™ Plasmid DNA Mediprep
GET™ Plasmid DNA Mediprep isolates super-coiled plasmid DNA from 25-200ml E. coli cultures. The extraction method is based on alkaline lysis of bacteria and uses our high affinity GET™ Plasmid Medi Columns to bind and purify plasmid DNA.
The extracted plasmid is ready for further DNA manipulation, such as restriction enzyme digestion, ligation, transformation, transfection and sequencing.
GET™ Plasmid DNA Mediprep Product Details | Order Online
GET™ Plasmid DNA Maxiprep
Isolates high quality plasmid DNA from 100-500ml E. coli cultures. The extraction method is based on alkaline lysis of bacteria and uses our high affinity GET™ Plasmid Maxi Columns to bind andpurify plasmid DNA.
The extracted plasmid is ready for further DNA manipulation, such as restriction enzyme digestion, ligation, transformation,transfection and sequencing.
The yield is on average 100-500µg/ prep depending on the volume of bacteria and plasmid copy number.
GET™ Plasmid DNA Maxiprep Product Details | Order Online
plasmid screening reagents
Plasmid Screeing Reagents
- IPTG
- HP Ampicillin™
- X-Gal
