2D Electrophoresis Reduction | Protein Research Products
protein reduction & alkylation
Unfolding and denaturation of protein molecules is often required for 2D gel electrophoresis application. Reduction of disulfide bridges and alkylation of thiols is necessary for complete denaturation of protein samples. G-Biosciences offers a selection of products for efficient reduction and alkylation of protein samples for 2D electrophoresis applications.
FOCUS™ Protein Reduction-Alkylation Kit
The FOCUS™ Reduction-Alkylation kit offers a simple two-step method for reduction and alkylation of protein samples for 2D gel analysis.
The disulfide bonds are reduced with a highly reactive and stable TCEP [Tris (2-carboxyethyl) phosphine] followed by blocking of the thiols by alkylation with iodoacetamide reagent preparation.
The kit is supplied with proprietary buffers and reagents necessary for an efficient reduction and alkylation of the disulfide bridges while minimizing reoxidation of the thiols.
FOCUS™ Protein Reduction-Alkylation Kit Product Details | Order Online
FOCUS™ Protein Reductant
A water soluble, odorless, non-toxic and stable TCEP [Tris (2-carboxyethyl) phosphine] reductant for protein reduction.
FOCUS™ Protein Reductant has improved stability and efficiency compared to DTT and reduces proteins over a wide range of pH, including lower acidic pH. FOCUS™ Protein Reductant completely reduces highly stable disulfide bonds in less than 5 minutes at room temperature.
FOCUS™ Protein Reductant is compatible with the alkylation of thiol groups for 2D analysis, as it does not compete with the alkylation reagent iodoacetamide, unlike DTT and other commonly used reductants.
The kit is supplied with a proprietary buffer necessary for efficient reduction of disulfide bonds, while minimizing reoxidation of the competing thiol pairs in protein samples.
FOCUS™ Protein Reductant Product Details | Order Online
FOCUS™ DTT [2M]
For those who prefer DTT as a reducing agent, we offer ultra pure proteomic grade DTT for protein reduction. FOCUS™ DTT is supplied as 5 vials containing dry powder to maintain the activity of the reducing agent. Add 1ml aliquot of water to give a 2M concentration, which is then added to your buffer solution to maintain protein reduction potential.
