Electrophoresis Products | Protein Research
protein gel stains
A selection of products for staining proteins in electrophoresis gels.
A major tool in the analysis of a species' proteome is the ability to separate the proteins by electrophoresis, 1D or 2D, and then the subsequent visualization of the proteins. G-Biosciences has developed a wide selection of electrophoresis gel stains for visualizing proteins that vary in sensitivity, staining time and reversibility.
For more information on protein gel stains, including a selection guide, read the technical handbook "Protein Electrophoresis":
Title: Protein Electrophoresis Handbook & Selection Guide
File Type: PDF
Author: G-Biosciences
LabSafe GEL Blue™
LabSafe GEL Blue™ is an enhanced protein stain that is based on Coomassie dye that offers unsurpassed sensitivity and rapid band visualization. LabSafe GEL Blue™ is supplied in a ready-to-use format, which is added directly to protein gels following electrophoresis after a brief wash step.
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FASTsilver™
A nanogram sensitive silver staining kit that produces crystal clear background and maximal sensitivity needed for critical analysis. A unique formulation that leaves the background clear and produces sharp images of protein bands. FASTsilver™ detects as little as 1ng BSA protein. FASTsilver™ does not use the protein modifier glutaraldehyde and therefore allows for the complete recovery of proteins and trypsin digested peptides. FASTsilver™ also stains nucleic acids and is able to detect as little as 0.3ng. The kit contains ready-to-use reagents for 25 mini gels and comes with a simple to follow 60-90 minute protocol.
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FOCUS™ FASTsilver™
FOCUS™ FASTsilver™ produces crystal clear backgrounds and maximal peptide recovery needed for critical analysis by mass spectrometry. FOCUS™ FASTsilver™ produces high quality silver staining without the use of glutaraldehyde and is supplied with a highly efficient silver ion removal reagent, SilverOUT™. SilverOUT™ removes silver ions, which permits complete peptide digestion and extraction of peptides for maximal recovery.
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Silver D-Stain™
The staining of gels with silver stains often results in over staining or unevenly stained gels. To save the trouble of repeating the electrophoresis, G-Biosciences offers Silver D-Stain™.
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RAPIDstain™
An ultra sensitive and ready-to-use protein stain based on Coomassie dye. No mixing or preparation is involved. No fixation step is needed. Simply rinse gel, add RAPIDstain™ and protein bands develop within 5-10 minutes, reaching a maximum visibility in an hour. Mini-gels can be stained in 10 minutes.
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Reversible Zinc Stain™
The Reversible Zinc Stain™ is a single step stain for the rapid detection of proteins resolved by PAGE (native gels or SDS denatured gels). No destaining is necessary.
The stain is based on the interaction of Zinc ions with polyacrylamide and proteins. The stain works by depositing a zinc metal precipitate in the gel which turns the gel opaque white,while the SDS coating on the proteins prevents the stains from binding to the proteins. A negative image of the gel is produced; clear protein bands are detected against a semi-opaque white polyacrylamide background. Protein bands are visualized in as little as 10-15 minutes.
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Reversible Copper Stain™
The Reversible Copper Stain™ is a single step stain for rapid detection of proteins resolved on SDS-PAGE. No destaining is necessary. The stain is based on the interaction of copper ions with polyacrylamide and proteins.
The stain works by depositing a copper metal precipitate in the gel, resulting in anopaque blue/green gel, while the SDS coating on the proteins inhibits copper ions from binding to the proteins. A negative image of the gel is produced; consisting of clear protein bands visualized against a semi-opaque blue/green polyacrylamide background.
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Glycoprotein Staining Kit
A modified silver stain based method allows sensitivity at nanogram levels (~1ng/band). Glycoprotein Staining Kit is a silver enhancement of traditional staining methods - it combines glycoprotein dye staining (GlycoBlue) with silver staining (FASTsilver™), which results in at least a two fold increase in sensitivity for detection of proteoglycans and glycoproteins. Glycoprotein Staining Kit stains both glycosylated and non-glycosylated proteins.
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Coomassie Brilliant Blue
Coomassie Brilliant Blue R-250/ G-250 are both offered as dry power form and convenient ready-to-use solutions. Destaining solution is also offered.
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