Sample Preparation Products | Protein Research Products
protein concentration systems
Many research applications require concentration of dilute protein solutions. Protein solutions may be concentrated by precipitation, chromatographic capture and elution, or by lyophilization. A disadvantage of lyophilization is non-protein agents are concentrated along with the protein and these may interfere with subsequent downstream applications.
G-Biosciences offers a selection of protein concentration kits suitable for concentrating proteins for running gels, raising antibodies, protein purification, protein assays and other application. Proteins concentrated with these kits are suitable for the majority of downstream applications.
UPPA-PROTEIN-Concentrate™
For Rapid Precipitation and Concentration Protein solutions, as dilute as 1ng/ml, can be quantitatively concentrated into a small volume. Protein concentration is not affected by the presence of common laboratory agents. The method involves mixing the protein solution with UPPA™ reagents that quantitatively precipitates protein. The precipitate is washed and suspended in a small volume of a suitable buffer. Recovery is generally >98%.
UPPA-PROTEIN-Concentrate™ Product Details | Order Online
OrgoSol-PROTEIN-Concentrate™
Suitable for those proteins that lose their biological activity when precipitated, such as enzymatic proteins. This kit uses OrgoSol Buffer - a proprietary solvent buffer for rapid and non-denaturing precipitation of protein. Simply mix protein solution with OrgoSol Buffer and incubate, which results in precipitation and recovery of the protein. The kit has been tested for the successful concentration of a wide selection of enzymatic proteins without the loss of biological activity. Recovery is generally 100%.
OrgoSol-PROTEIN-Concentrate™ Product Details | Order Online
Column-PROTEIN-Concentrate™
Suitable for larger volumes of dilute protein solution or when other methods don’t concentrate protein. This kit is based on chromatography immobilization and elution of protein at any pH, followed by spin-elution of the protein in a small volume of buffer. The kit has been tested for concentration of a wide variety of proteins. This method is gentle and protects protein from denaturation during the concentration process.
Column-PROTEIN-Concentrate™ Product Details | Order Online
