Protein Research Products | Antibodies Production & Purification

carrier proteins

The use of peptides for the generation of antibodies against specific peptides has become an essential tool in proteomic research.

A crucial step in using peptides as antigens is their coupling to carrier proteins. Peptides are designed to be good epitopes by themselves; however they fail to elicit a strong antibody response. The reason for this is that the epitopes are able to bind to the B-cells, but are too small to contain additional motifs required to associate with class II molecules and T-cell receptors. The carrier proteins, once coupled to the peptides, provide these additional motifs and generate a strong antibody response.

The most common carrier proteins are bovine serum albumin (BSA; 67kDa) and keyhole limpet hemocyanin (KLH; 4.5x105-1.3x107Da). In addition, we offer HyperCarrier™, a cationized BSA that has been treated with ethylene diamine, which substitutes anionic carboxyl groups with cationic aminoethyl-amide groups. The use of cationized BSA is known to elicit greater immunogenic response compared to normal BSA.

The carrier proteins are coupled to haptens, including peptides and small proteins, Online Storewith the use of protein cross-linkers. All three carrier proteins are also offered as activated carrier proteins, named as ActiveHOOK™. ActiveHOOK™ carrier proteins have been pre-activated with the heterobifunctional protein cross linker sulfoSMCC and therefore are ready for the addition of cysteine containing peptides or proteins for a simple one step reaction.
Carrier Proteins Product Details | Order Online

Bovine Serum Albumin (BSA)
Bovine serum albumin is a single polypeptide of 67kDa and consists of 59 lysine residues, of which 30-35 have primary amines that can react with crosslinkers for the Online Storecoupling of peptides.

OneQuant™ BSA
The BSA carrier protein is also available as our single use OneQuant™ vials to save time and money. Supplied as 8 x 2mg vials.
Bovine Serum Albumin (BSA) Product Details | Order Online

Keyhole Limpet Hemocyanin (KLH)
Keyhole limpet hemocyanin is a widely used carrier protein due to its large molecular mass (4.5x105-1.3x107Da aggregates) and large number of primary amines that can Online Storereact with cross-linkers for the coupling of peptides.

OneQuant™ KLH
The KLH carrier protein is also available as our single use OneQuant™ vials to save time and money. Supplied as 8 x 2mg vials.
Keyhole Limpet Hemocyanin (KLH) Product Details | Order Online

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