Protein Research Products | Antibodies Production & Purification
peptide coupling kits
The use of peptides for the generation of antibodies against specific peptides has become an essential tool in proteomic research.
A crucial step in using peptides as antigens is their coupling to carrier proteins. Peptides are designed to be good epitopes by themselves; however they fail to elicit a strong antibody response. The reason for this is that the epitopes are able to bind to the B-cells, but are too small to contain additional motifs required to associate with class II molecules and T-cell receptors.
The carrier proteins, once coupled to the peptides, provide these additional motifs and generate a strong antibody response.
HOOK™ Peptide Coupling Kit (Sulfhydryl Reactive)
A common practice in the design of peptides is the addition of a cysteine residue at the N- or C-terminus of the peptide, which can be used for coupling to carrier proteins.
This kit is designed for the coupling of peptides to carrier proteins, utilizing a sulfhydryl group in the peptide. This kit exploits the chemical cross-linker sulfoSMCC (Sulfosuccinimidyl 4-(N-maleimidomethyl) cyclohexane-1-carboxylate) to couple peptides through their sulfhydryl groups, found on cysteine side chains, to the primary amines on the carrier proteins.
HOOK™ Peptide Coupling Kit (Sulfhydryl Reactive) Product Details | Order Online
HOOK™ Peptide Coupling Kit (Amine Reactive)
This kit is designed for the coupling of peptides to carrier proteins, utilizing the primary amines and carboxyl groups of the peptides and carrier proteins. This kit utilizes the chemical heterobifunctional crosslinker EDC (1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride) to couple peptides to carrier proteins. EDC first reacts with the carboxyl groups, producing an amine reactive intermediate, O-acylisourea that rapidly reacts with the amine groups of the peptide/protein.
HOOK™ Peptide Coupling Kit (Amine Reactive) Product Details | Order Online
