Protein Research Products | Antibodies Production & Purification
Purification Resins & Systems
A selection of Affinity resins for the purification of antibodies.
Immobilized Protein A
Immobilized Protein A is for binding the constant domains of immunoglobulin (Ig) molecules. Protein A is coupled to agarose beads by a proprietary coupling method that provides high coupling efficiency for immunoglobulins and minimal protein A leaching. Our immobilized protein A has a high binding capacity (18-43mg/ml resin).
Immobilized Protein A Product Details | Order Online
Immobilized Protein G
Immobilized protein G is for binding the constant domains of immunoglobulin (Ig) molecules. Protein G is a modified form of Streptococcal group G so that it does not bind to albumin. Protein G is coupled to 4% cross-linked agarose beads by a proprietary coupling method that provides high coupling efficiency for Ig and minimal protein G leaching. Our immobilized protein G has a high binding capacity (>20mg/ml resin).
Immobilized Protein G Product Details | Order Online
HOOK™ Activated Agarose (Amine Reactive)
For the generation of peptide and protein affinity columns, for the purification of antibodies and for the discovery of important interacting proteins and cofactors. G-Biosciences offers activated agarose for the coupling of peptides and proteins to agarose through their primary amines.
The amine reactive HOOK™ Activated Agarose is 6% agarose that has been activated to generate reactive aldehyde groups. The aldehyde groups of the agarose react spontaneously with primary amines, located at the N-terminus of proteins or in lysine residues, to form intermediate Schiff Base complexes. These, in turn, are selectively reduced by reductive amination to form stable amine linkages between the agarose and the ligand.
HOOK™ Activated Agarose (Amine Reactive) Product Details | Order Online
HOOK™ Activated Agarose (Carboxy/Sulfhydryl Reactive)
For the generation of peptide and protein affinity columns, for the purification of antibodies and for the discovery of important interacting proteins and cofactors. G-Biosciences offers activated agarose for the coupling of peptides and proteins to agarose through their carboxy or sulfhydryl groups.
The carboxy/sulfhydryl reactive HOOK™ Activated Agarose is 6% agarose beads that have been activated by the addition of an aminoethyl group.Proteins and peptidesare coupled to the agaroses amine group via the use of common protein crosslinkers, such as EDC (Cat. # BC25) or sulfoSMCC (Cat. # BC23). EDC will couple protein/peptide carboxyl groups to the agarose, whereas sulfoSMCC will react with the sulfhydryl residues of the proteins/peptides.
HOOK™ Activated Agarose (Carboxy/Sulfhydryl Reactive) Product Details | Order Online
