Protein Research Products | Purification & Chromatography
Affinity Purification Resins & Systems
For the separation & purification of affinity tagged proteins & antibodies.
The separation and purification of proteins has been a major challenge for researchers in the early days of proteomic research. The advances in affinity chromatography have enabled researchers to purify large quantities of highly pure proteins for a multitude of analysis techniques, including crystallography, protein:protein studies and in-vitro assays. Affinity chromatography works by binding a protein, via a reversible interaction, to a specific ligand that is prebound to a solid chromatographic support. The protein(s) are first bound to the column in a buffer that supplies conditions optimal for binding. Unbound, non-specific material is washed away and the protein(s) of interest are then eluted by changing the buffering conditions to induce desorption from the solid support.
G-Biosciences supplies two main groups of affinity resins. The first group is for the separation and purification of affinity tagged proteins and the second group is for the binding of immunoglobulin molecules. A common practice in today’s research is the use of molecular biology to clone our protein(s) of interest into a vector that adds a specific tag to the protein. The most versatile and common tags used are glutathione S-transferase (GST), a 6x histidine motif (His-tag), and the calmodulin binding peptide (CBP).
Glutathione Resin
Glutathione Resin is designed for the affinity purification of proteins with a glutathione S-transferase (GST) tag. The resin consists of reduced glutathione (GSH) covalently coupled to 4% cross-linked agarose, via a 10-carbon spacer arm. The resin has a 
binding capacity of ~8mg GST/ml resin. Supplied as slurry in 20% ethanol.
Supplied as a kit that includes 10ml resin, 100ml binding/ wash buffer, 100ml elution buffer, 5 micro spin and 5 medi gravity flow columns.
The resin, columns and buffers are available separately..
Glutathione Resin Product Details | Order Online
Nickel Chelatin Resin
Immobilized metal affinity chromatography (IMAC) resin utilizing nickel (Ni2+) for the purification of 6x histidine tagged proteins.
This resin binds to six histidine residues (6x His), a common tag used in protein purification. The resin consists of iminodiacetate coupled to 6% cross-linked agarose beads. The iminodiacetate binds divalent nickel ion with a capacity of 20-40μmoles Ni2+/ml resin.
Nickel Chelation Resin Product Details | Order Online
Calmodulin Resin
Calmodulin Resin for the affinity purification of calmodulin binding proteins (CBP), including recombinant proteins with a CBP tag and calmodulin-regulated proteins in eukaryotic cells. The resin is 4% agarose coupled to calmodulin and has a ligand density of approximately 1mg calmodulin/ml resin.
Calmodulin Resin Product Details | Order Online
Immobilized Protein A
Immobilized Protein A is for binding the constant domains of immunoglobulin (Ig) molecules (see Figure 1). Protein A is coupled to agarose beads by a proprietary coupling method that provides high coupling efficiency for immunoglobulins and minimal protein A leaching. Our immobilized protein A has a high binding capacity (18-43mg/ml resin).
Immobilized Protein A Product Details | Order Online
Immobilized Protein G
Immobilized protein G is for binding the constant domains of immunoglobulin (Ig) molecules (see Figure 1). Protein G is a modified form of Streptococcal group G so that it does not bind to albumin. Protein G is coupled to 4% cross-linked agarose beads by a proprietary coupling method that provides high coupling efficiency for Ig and minimal protein G leaching. Our immobilized protein G has a high binding capacity (>20mg/ml resin).
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Streptavidin Resin
Streptavidin is a tetrameric protein and in many respects is similar to avidin except that it has no carbohydrate and has a slightly lower molecular weight of about 60kDa. The solubility of streptavidin (isoelectric pH5) in aqueous buffer is much lower than avidin, but the binding of streptavidin to biotin is similar to that of avidin. The advantage of streptavidin is that the lack of carbohydrates significantly reduces the amount of non-specific binding.
The Steptavidin Resin is designed for the single step affinity purification of proteins and antibodies with a biotin tag. The resin consists of steptavidin coupled to ~4% cross-linked agarose, via a 15 carbon spacer arm.
Streptavidin Product Details | Order Online
HOOK™ Activated Agarose (Amine Reactive)
For the generation of peptide and protein affinity columns, for the purification of antibodies and for the discovery of important interacting proteins and cofactors. G-Biosciences offers activated agarose for the coupling of peptides and proteins to agarose through their primary amines.
The amine reactive HOOK™ Activated Agarose is 6% agarose that has been activated to generate reactive aldehyde groups. The aldehyde groups of the agarose react spontaneously with primary amines, located at the N-terminus of proteins or in lysine residues, to form intermediate Schiff Base complexes. These, in turn, are selectively reduced by reductive amination to form stable amine linkages between the agarose and the ligand.
HOOK™ Activated Agarose (Amine Reactive) Product Details | Order Online
HOOK™ Activated Agarose (Carboxy/Sulfhydryl Reactive)
For the generation of peptide and protein affinity columns, for the purification of antibodies and for the discovery of important interacting proteins and cofactors. G-Biosciences offers activated agarose for the coupling of peptides and proteins to agarose through their carboxy or sulfhydryl groups.
The carboxy/sulfhydryl reactive HOOK™ Activated Agarose is 6% agarose beads that have been activated by the addition of an aminoethyl group. Proteins and peptides are coupled to the agaroses amine group via the use of common protein crosslinkers, such as EDC (Cat. # BC25) or sulfoSMCC (Cat. # BC23). EDC will couple protein/peptide carboxyl groups to the agar ose, whereas sulfoSMCC will react with the sulfhydryl residues of the proteins/peptides. For further information on the crosslinkers see the Cross Linking & Modification section.
HOOK™ Activated Agarose (Carboxy/Sulfhydryl Reactive) Product Details | Order Online
other purification & chromatography products:
- Protein Purification Optimization Systems
- Recombinant Protein Solubilization
- Purification Accessories
