Protein Research Products | Purification & Chromatography
recombinant protein solubilization
The expression of recombinant proteins is a popular and routinely used technique in protein studies. The expression of recombinant proteins often has one drawback and that is the recombinant proteins aggregate and form inclusion bodies, especially when expressed at high levels. The aggregated proteins are difficult to solubilize, due to the nature of aggregates, however G-Biosciences offers a selection of products for dealing with the range of issues involved with solubilizing and recovering active proteins from inclusion bodies.
IBS™ Buffer
The IBS™ buffer is specifically developed for solubilization of inclusion bodies. Simple to use protocol as inclusion bodies are suspended in IBS™ Buffer, where they readily dissolve releasing the proteins of interest. Once the inclusion bodies are solubilized, the sample is ready for further analysis and other downstream applications.
IBS™ Buffer Product Details | Order Online
IBS-HP™ Buffer
Solubilization of Hydrophobic Proteins from Inclusion Bodies We offer IBS-HP™ Buffer for the solubilization of inclusion bodies containing highly hydrophobic proteins. The IBS-HP™ Buffer contains optimized concentration of SDS, a buffering agent and urea. After solubilization of inclusion bodies, free SDS may be quenched or removed by competing with a non-ionic detergent.
IBS™ Buffer Product Details | Order Online
Disposable Columns
Affordable, disposable columns available in three convenient sizes.
Disposable Columns Product Details | Order Online
other purification & chromatography products:
- Protein Purification Optimization Systems
- Affinity Purification Resins & Systems
- Purification Accessories
